The article deals with the regional specifics of student teachers’ professional pedagogical orientation. The importance of considering macrosocial context of the formation of student teachers’ professional orientation is noted. The results of empirical research of the professional orientation level, its motivational and value components among student-teachers of three regions: Pridnestrovie, Kishinev municipality and Orekhovo-Zuevo municipality are presented. It is argued that student-teachers in various regions differ in the professional orientation level (it is lower among Chisinau students in comparison to those from Pridnestrovie and Orekhovo-Zuevo), whereas motivational and value components also vary: the professional orientation of student-teachers from Pridnestrovie, a region with undefined political status, is characterized by predominance of external motivation and more pronounced individualistic tendencies (in comparison to students from culturally similar regions).